Figure 2.

(a) Kinetic traces (λ_ex 365 nm, λ_em 460 nm) and Michaelis–Menten plot (inset) for the hydrolysis of pro-fluorophore 2 (20 μM→9.8 nM) by PLE (2.5 μg/mL); k_cat/K_M = 2.5 × 10⁵ M⁻¹s⁻¹ and K_M = 8.2 μM. (b) Kinetic traces (λ_ex 586 nm, λ_em 620 nm) and Michaelis–Menten plot (inset) for the hydrolysis of pro-fluorophore 4 (2.5 μM→4.9 nM) by PLE (2.5 μg/mL); apparent k_cat/K_M = 1.2 × 10⁵ M⁻¹s⁻¹ and apparent K_M = 0.14 μM.