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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: J Mol Biol. 2009 Sep 3;394(3):391–409. doi: 10.1016/j.jmb.2009.08.069

Figure 5. Effects of inducer concentrations on BiFC complex formation.

Figure 5

Cells that expressed (A) FKBP-YN and FRB-YC (B) FKBP-VN and FRB-YC or (C) FKBP-VyN and FRB-YC were treated different concentrations of rapamycin (purple) or AP21967 (orange) for 4 hours at 37 °C. The cells were fixed and their fluorescence intensities were measured by flow cytometry. The mean ratio of BiFC to CFP fluorescence was plotted as a function of inducer concentration. The data shown are representative of 3–6 independent experiments for each inducer and combination of fusion proteins.