Table 1.

Thermodynamic parameters for the reversible association of aminobenzyl-DOTA chelates to Antibody 2D12.5 at 30 °C and 37 °C. (Standard deviations in parentheses.)^a

Chelate	T, °C	KA, M−1	ΔG, kcal/mol	ΔH, kcal/mol	ΔS, cal/mol-K	KD, M
ABD(Co)	30	8.2(±0.9) ×105	−8.2(±0.1)	−12.2(±0.4)	−13.2(±1.3)	1.2(±0.1) ×10−6
ABD(Co)	37	7.1(±0.4) ×105	−8.3(±0.1)	−12.4(±4.8)	−13.3(±4.8)	1.4(±0.1) ×10−6
ABD(Y)	30	3.3(±2.1) ×108	−11.7(±0.4)	−18.2(±0.5)	−21.5(±2.9)	3.1(±2.0) ×10−9
ABD(Y)	37	5.2(±0.9) ×108	−12.4(±0.2)	−20.1(±1.2)	−24.9(±4.1)	2.0(±0.4) ×10−9

^aData from ITC experiments in Figure 1 and Figure 2.