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. Author manuscript; available in PMC: 2011 Apr 16.
Published in final edited form as: J Mol Biol. 2010 Feb 26;397(5):1276–1286. doi: 10.1016/j.jmb.2010.02.042

Figure 1.

Figure 1

Cryo-EM analysis of wt LTag and LTag108-627 double hexamers at the SV40 origin of replication. (a) Sequence scheme of wt LTag and LTag108-627. The N-terminal region is indicated with a red line. The plot indicates the intrinsic ordered (green) or disordered (red) nature of LTag sequences predicted by the server FoldIndex© 61. (b) Reference-free 2D averages of wt and mutant LTag Cryo-EM images. Red lines indicate the orientation of the central region of each hexamer (c) Cryo-EM maps of wtLTag-ori complex structures in the parallel and displaced conformations. Annotations indicate each of the two hexamers (Hex 1 and 2) and the C-terminal (C-ter) and central regions. (d) Cryo-EM map of the single class found for LTag108-627 (e) A predicted dsDNA structure of the SV40 ori as calculated by the web server bent.it© 62 is superimposed to LTag108-627 map. The central perfect palindrome is depicted in green (P1, P4) and yellow (P2, P3) and the flanking regions in red (A/T-rich region) and in purple (EP region). Arrows point towards the edges of the flanking regions, where a marked bend is predicted.