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. Author manuscript; available in PMC: 2011 Apr 16.
Published in final edited form as: J Mol Biol. 2010 Feb 26;397(5):1276–1286. doi: 10.1016/j.jmb.2010.02.042

Figure 3.

Figure 3

Wt LTag C-terminal domain structures at the SV40 ori. (a) Diagram of LTag double hexamer assembled onto an asymmetric DNA probe, which extends beyond the LTag-occluded region at the AT end (blue). The probe used for wt LTag (dark blue) extends 7 nucleotides more than for LTag108-627 (light blue). The central perfect palindrome is depicted in yellow and the flanking regions in red (A/T-rich region) and in purple (EP region). (b) Raw images of LTag double hexamer complex. The hexamers with the protruding DNA have been oriented to the upper part of the image. (c) Class averages with a clear protruding DNA obtained by classification with a combination of MLF2D 38 and KerDenSOM 53. (d) Maps of wt LTag C-terminal regions bound to A/T-rich (AT-Cter) and EP (EP-Cter) regions displayed at the same threshold and their difference map. Central slices of each map are shown as insets. In the difference maps, red mesh shows extra density in AT-Cter and green mesh, extra density in EP-Cter. Bars represent 10nm.