Fig. 6.
Temporal expression of D15A in the spinal cord after in vivo gene delivery. Lentiviral delivery resulted in robust and stable gene expression for up to 4 weeks post-injection. At 4 weeks post-injection, the D15A levels were significantly higher than both adenoviral infected groups (P < 0.001). In contrast, adenoviral infection showed significant downregulation of D15A at 2 (P < 0.001) and 4 weeks (P < 0.05) post-infection. Treatment of animals with mAb OX-22/OX-38 successfully reduced the downregulation of adenoviral-delivered D15A expression compared to the non-OX22/OX38 treated groups that was significantly lower (P < 0.001) than lentivirus and adenovirus + mAb groups at 4 weeks. Data represent the mean ± SD (n = 5/experimental group).