Skip to main content
. Author manuscript; available in PMC: 2011 Apr 30.
Published in final edited form as: Circ Res. 2010 Mar 11;106(8):1394–1403. doi: 10.1161/CIRCRESAHA.109.210286

Figure 5. Matrix-specific signaling in oscillatory flow.

Figure 5

BAECs plated on matrigel or fibronectin were exposed to oscillatory flow for 18 hours. Activation of (A) PAK and (A,B) NF-κB were determined by Western blotting with phospho-specific antibodies as previously described. n = 3–4. (C) Cells were fixed and NF-κB nuclear translocation was determined as in previous figures. Representative images are shown. n = 3 (D) PKA activity was determined using the GST-PKI affinity beads. Active PKA in the pulldown was normalized to total PKA in the lysate. n = 3. Statistical method was student’s T-test.