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. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: J Cell Biochem. 2005 Aug 15;95(6):1108–1119. doi: 10.1002/jcb.20473

Fig. 3.

Fig. 3

Nicotine decreased TGF-β1-induced p38-MAPK activity. HGFs were pretreated with different concentrations of nicotine (0.01, 0.1, or 1 mM) for 2 h (black bars) and then treated with TGF-β1 (0.5 ng/ml) with or without nicotine (gray bar). Phosphor-p38 MAPK was detected by Western blots as previously described. Densitometric analysis showed that TGF-β1 treatment up-regulated p38-MAPK activity compared to untreated cells (NT open bar), while nicotine inhibited TGF-β1-induced p38-MAPK activity (black bars). Error bars equal the standard deviation between three experiments.