Figure 7.

Axonal transport defects in DMob4 mutants. A, B, DMob4 mutant third-instar larvae have a tail-flip phenotype (B) compared with controls (A). C–F, Peripheral nerves of third-instar larvae coimmunolabeled for Syt-1 (red) and DMob4 (green). Blockade of axonal transport in DMob4^EYΔL3/Df(2R)42 (null) and DMob4^EYΔL307/Df(2R)42 (hypomorph) mutants results in accumulation of Syt-1 in large aggregates along the length of peripheral nerves (E, F). Axonal transport defects in DMob4^EYΔL3 mutants can be rescued with the ectopic expression of UAS–DMob4 in neurons using the ElavGal4 driver (D). G, Quantification of Syt-1 immunoreactive inclusions present in peripheral nerves of DMob4 mutants, controls, and rescued animals. Scale bar, 20 μm.