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. 2010 Apr 23;6(3):213–224. doi: 10.7150/ijbs.6.213

Figure 4.

Figure 4

The HSPB1 expression in normal kidney, RO and chromophobe RCC. (A) The presence of HSPB1 isoforms within five spots situated along a line in chromophobe RCC. The same zoomed areas of 2D-PAGE images shows the position of two spots in the dotted square. (B) Immunodetection of the protein with anti-HSP27 (HSPB1) antibody. Western blot analysis confirmed an increased expression of the HSPB1 protein in chromophobe RCCs in comparison to ROs. ACTB was used as a control. (C) A significantly increased mRNA level in chromophobe RCC as detected by microarray-based gene expression analysis (probe set 201841_s_at). (D) Specific overexpression of the HSPB1 gene in chromophobe RCCs in comparison to ROs has been confirmed by real-time PCR as well.

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