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. 2010 Mar 5;153(1):260–272. doi: 10.1104/pp.110.154443

Table II. Diurnal dynamics of identified enzyme parameters represented in the model.

Values of Km and Ki are given in mm. The unit of maximum enzyme activity (Vmax) is μmol h−1 g−1 fresh weight. Asterisks indicate significant differences from the wild type (* P < 0.01, ** P < 0.001).

Genotype Reaction Parameter Time
9 am 1 pm 5 pm 9 pm 1 am 5 am
Col-0 rSP→Suc Vmax 27.9 ± 1.8 27.1 ± 1.4 21.9 ± 2.2 12.2 ± 1.3 4.8 ± 0.3 0.4 ± 0.2
Km: 0.53 ± 0.082
rSuc→Hex Vmax 68.3 ± 8.1 155.5 ± 12.0 141.6 ± 12.1 99.9 ± 7.5 70.2 ± 5.5 68.0 ± 6.9
Km: 11.7 ± 1.21
Ki,Glc: 0.01 ± 0.001
Ki,Fru: 0.78 ± 2.29
rFru→SP Vmax 3.5 ± 0.5 3.6 ± 0.6 7.9 ± 1.3 8.0 ± 1.3 8.1 ± 1.4 4.1 ± 0.5
Km: 0.8 ± 0.075
Ki: 1.97 ± 0.31
rGlc→SP Vmax 6.1 ± 1.0 6.2 ± 1.7 12.4 ± 2.6 12.9 ± 2.8 10.27 ± 2.3 4.83 ± 0.9
Km: 0.1 ± 0.02
Ki: 0.14 ± 0.03
inv4 rSP→Suc Vmax 23.1 ± 1.0* 21.9 ± 1.0* 17.0 ± 1.2* 12.3 ± 1.3 3.0 ± 1.1* 3.9 ± 6.8
Km: 0.27 ± 0.02
rSuc→Hex Vmax 12.9 ± 0.2** 12.9 ± 0.2** 15.0 ± 0.001** 17.0 ± 1.8** 11.8 ± 1.3** 11.0 ± 0.001**
Km: 8.12 ± 2.74
Ki,Glc: 0.07 ± 0.06
Ki,Fru: 1.9 ± 4
rFru→SP Vmax 5.8 ± 1.8* 4.4 ± 1.2* 9.5 ± 2.8 8.8 ± 2.6 7.1 ± 2.5* 4.9 ± 1.6
Km: 1.17 ± 0.27
Ki: 2.16 ± 0.47
rGlc→SP Vmax 6.0 ± 1.8 5.2 ± 1.5* 9.5 ± 2.8* 7.9 ± 2.4* 6.7 ± 2.3* 4.5 ± 1.5
Km: 0.08 ± 0.02
Ki: 0.147 ± 0.016