Table II. Diurnal dynamics of identified enzyme parameters represented in the model.
Genotype | Reaction | Parameter | Time |
|||||
9 am | 1 pm | 5 pm | 9 pm | 1 am | 5 am | |||
Col-0 | rSP→Suc | Vmax | 27.9 ± 1.8 | 27.1 ± 1.4 | 21.9 ± 2.2 | 12.2 ± 1.3 | 4.8 ± 0.3 | 0.4 ± 0.2 |
Km: 0.53 ± 0.082 | ||||||||
rSuc→Hex | Vmax | 68.3 ± 8.1 | 155.5 ± 12.0 | 141.6 ± 12.1 | 99.9 ± 7.5 | 70.2 ± 5.5 | 68.0 ± 6.9 | |
Km: 11.7 ± 1.21 | ||||||||
Ki,Glc: 0.01 ± 0.001 | ||||||||
Ki,Fru: 0.78 ± 2.29 | ||||||||
rFru→SP | Vmax | 3.5 ± 0.5 | 3.6 ± 0.6 | 7.9 ± 1.3 | 8.0 ± 1.3 | 8.1 ± 1.4 | 4.1 ± 0.5 | |
Km: 0.8 ± 0.075 | ||||||||
Ki: 1.97 ± 0.31 | ||||||||
rGlc→SP | Vmax | 6.1 ± 1.0 | 6.2 ± 1.7 | 12.4 ± 2.6 | 12.9 ± 2.8 | 10.27 ± 2.3 | 4.83 ± 0.9 | |
Km: 0.1 ± 0.02 | ||||||||
Ki: 0.14 ± 0.03 | ||||||||
inv4 | rSP→Suc | Vmax | 23.1 ± 1.0* | 21.9 ± 1.0* | 17.0 ± 1.2* | 12.3 ± 1.3 | 3.0 ± 1.1* | 3.9 ± 6.8 |
Km: 0.27 ± 0.02 | ||||||||
rSuc→Hex | Vmax | 12.9 ± 0.2** | 12.9 ± 0.2** | 15.0 ± 0.001** | 17.0 ± 1.8** | 11.8 ± 1.3** | 11.0 ± 0.001** | |
Km: 8.12 ± 2.74 | ||||||||
Ki,Glc: 0.07 ± 0.06 | ||||||||
Ki,Fru: 1.9 ± 4 | ||||||||
rFru→SP | Vmax | 5.8 ± 1.8* | 4.4 ± 1.2* | 9.5 ± 2.8 | 8.8 ± 2.6 | 7.1 ± 2.5* | 4.9 ± 1.6 | |
Km: 1.17 ± 0.27 | ||||||||
Ki: 2.16 ± 0.47 | ||||||||
rGlc→SP | Vmax | 6.0 ± 1.8 | 5.2 ± 1.5* | 9.5 ± 2.8* | 7.9 ± 2.4* | 6.7 ± 2.3* | 4.5 ± 1.5 | |
Km: 0.08 ± 0.02 | ||||||||
Ki: 0.147 ± 0.016 |