Figure 3.

Schematic diagram of an improved version of the MISSA vectors as well as recombination reaction events based on these vectors. A, The main differences of the improved version of the donor vectors, pLC series and pRG series compared with the original ones (Fig. 2), are that two sorts of antibiotic resistance genes are used and the genes are inserted between the loxP and attL1/attR2 sites to enhance the selection efficiency of recombination clones. B and C, The recombination reactions are similar to those in Figure 2 except that different selection media are used for recombination clones. Two representative rounds of recombination are shown. Ap, Ampicillin; Cm, chloramphenicol; Glu, Glc; Gm, gentamycin; Kn, kanamycin. [See online article for color version of this figure.]