Table IV. Functional testing of Z-ISO in E. coli accumulating 9,15,9′-tri-cis-ζ-carotene.

E. coli carrying bacterial crtE (encoding GGPPS), crtB (encoding PSY), and maize PDS accumulate 9,15,9′-tri-cis-ζ-carotene and were additionally transformed with the genes denoted or empty vector (pCOLADuet-1) as a control. Numbers correspond to percentage of total ζ-carotene based on peak area relative to total peak area of six ζ-carotene peaks.

Added Genes	9,15,9′-Tri-cis-ζ-carotene	9,9′-Di-cis-ζ-carotene
Empty vector	34.40 ± 1.05	33.02 ± 0.64
AtZ-ISO1.1	8.91 ± 2.44	52.81 ± 3.95
ZmZ-ISO	6.09 ± 1.26	67.57 ± 3.01
AtZ-ISO1.1M	33.53 ± 2.85	36.64 ± 4.60
AtZ-ISO1.2	33.14 ± 2.26	32.08 ± 3.07
Bacterial NnrU	36.83 ± 3.75	33.38 ± 1.60