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. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: Neurotox Res. 2009 May 28;16(2):148–159. doi: 10.1007/s12640-009-9064-7

Fig. 3.

Fig. 3

The effect of 1-R-aminoindan on 75 mM ethanol-induced nuclear accumulation of GAPDH/transcriptional activator complex. The interaction of GAPDH with TIEG2 (an MAO B transcriptional activator) in nucleus was determined by Co-IP assay. a Nuclear proteins isolated from U-118 MG or SH-SY5Y cells which were treated without ethanol (lane 1) or with ethanol (lane 2) or with both ethanol and 1-R-aminoindan (lane 3) for 72 h were immunoprecipitated by incubating with anti-TIEG2 antibody. Protein Co-IP with TIEG2 was analyzed by Western blot with anti-GAPDH antibody (lanes 1–3). b Quantitative analysis. The relative intensity of TIEG2-bound GAPDH band was quantified by Kodak Imaging Analysis System. The GAPDH levels were increased by ethanol but reduced by 1-R-aminoindan. Data represent the mean ±SD of four independent experiments. *P < 0.001 compared with respective ethanol-treated group (without 1-R-aminoindan)