Effects of GAPDH and 1-R-aminoindan on ethanol-induced MAO B mRNA level, catalytic activity, generation of H2O2, and cell proliferation rate (MTT assay). GAPDH- or pcDNA3.1-overexpressed U-118 MG cells were treated by 75 mM of ethanol without or with 1-R-aminoindan (1 μM) as indicated in the figure for 48 h (for mRNA level) or 72 h (for MAO B catalytic activity, generation of H2O2 or MTT assay). a MAO B mRNA levels, b MAO B catalytic activity, c generation of H2O2, and d cell proliferation rate were determined, respectively. Controls were untreated cells (ethanol 0 mM) which were taken as 1 in (a) or taken as 100% in (c) and (d). Data represent the mean ± SD of three independent experiments. *P < 0.01 compared with pcDNA3.1-transfected cells in each ethanol treatment group (lane 5). #P < 0.02 compared with GAPDH-overexpressed cells in each ethanol treatment group (lane 7)