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. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: Neurotox Res. 2009 May 28;16(2):148–159. doi: 10.1007/s12640-009-9064-7

Fig. 5.

Fig. 5

Effects of TIEG2-knockdown on ethanol-induced MAO B mRNA level, generation of H2O2, and cell proliferation rate (MTT assay). GAPDH-overexpressed U-118 MG cells were transfected with TIEG2-siRNA or control-siRNA. a After transfected with siRNA for 3 days, TIEG2 protein from each cell line was evaluated by Western blot analysis. b After transfected with siRNA for 24 h, cells were treated with 75 mM ethanol, and then harvested after 2 days for determining MAO B mRNA level, c the production of H2O2 and d cell proliferation rate/viability. Controls were untreated cells (ethanol 0 mM) which were taken as 1 in (b) or taken as 100% in (c) and (d). Data represent the mean ± SD of three independent experiments.

*P < 0.02 compared to control-siRNA-transfected cells in each ethanol treatment group (lane 3)