Figure 5.
Metformin increases the translocation of LKB1 from nucleus into cytosol in HUVEC. A, Immunocytochemical staining of translocation of LKB1 from nucleus to the cytosols caused by metformin in HUVEC. B and C, Metformin (Met) also increases the amount of LKB1 in the cytosol while it decreases LKB1 in the nuclei in HUVEC. The blot is a representative of 5 blots obtained from 5 independent experiments. Con indicates control. D and E, Metformin increases the serine 428 phosphorylation of LKB1 in both nucleus and cytosol. The blot is a representative of 3 blots obtained from 3 independent experiments.