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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: J Am Chem Soc. 2010 May 5;132(17):6183–6193. doi: 10.1021/ja100710j

Figure 10.

Figure 10

Deactivation of antisense oligonucleotide function in mammalian tissue culture. A) The hairpin antisense agent R6 is active in the absence of light irradiation, silencing the DsRed gene. B) Irradiation of cells removes the caging groups, enabling the formation of a hairpin that blocks the antisense agent from recognizing the mRNA transcript. Inactivation of the antisense agent then allows for DsRed expression. GFP/DsRed (left) corresponds to an overlay of the GFP and DsRed images, while only the DsRed channel is shown on the right. Scale bar = 200 μm.