Skip to main content
. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: J Am Chem Soc. 2010 May 5;132(17):6183–6193. doi: 10.1021/ja100710j

Figure 11.

Figure 11

Measurement of DsRed and GFP fluorescence to quantify intracellular antisense activity. Transfections were performed as previously described, and the cells were either irradiated (2 min, 365 nm, 25 W), or not exposed to UV irradiation. After 48 hours fluorescence was measured on a Molecular Devices Gemini EM microplate spectrofluorimeter. Relative fluorescence (DsRed/GFP) normalized to a transfection in the absence of DNA is shown. Error bars represent standard deviations from three independent experiments. The mRNA translational suppression of ~30% with the non-caged analog R1 is in accordance with previous reports.28