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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: J Am Chem Soc. 2010 May 5;132(17):6183–6193. doi: 10.1021/ja100710j

Figure 3.

Figure 3

Light-activated DNAzyme. The caged 10-23 DNAzyme D2 (40 nM) binds to its complementary RNA (4 nM), but has no catalytic activity due to incorporation of the caged thymidine 3 at the crucial position T12 in the catalytic core. Irradiation at 365 nm removes the caging group, activates the DNAzyme, and induces RNA cleavage at a Mg2+ concentration of 100 mM (15 mM Tris buffer, pH 8.2). The RNA degradation was imaged by gel-separation of a 32P labeled RNA substrate.