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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: J Am Chem Soc. 2010 May 5;132(17):6183–6193. doi: 10.1021/ja100710j

Figure 4.

Figure 4

Time course of RNA cleavage by the DNAzymes D1 (non-caged; 40 nM) and D2 (caged; 40 nM) under different irradiation and re-folding conditions. All reactions were performed with 4 nM 32P-labeled RNA substrate (10 mM MgCl2, pH 8.2, 15 mM Tris buffer). RNA cleavage was assessed via the removal of aliquots of the sample at given time points, followed by PAGE analysis (see Figure 3) and quantification of the radioactively labeled RNA substrate with ImageQuant. All experiments were conducted in triplicate and the error bars represent standard deviations.