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. Author manuscript; available in PMC: 2011 May 5.
Published in final edited form as: J Am Chem Soc. 2010 May 5;132(17):6183–6193. doi: 10.1021/ja100710j

Figure 8.

Figure 8

Terminal hairpins introduced on the DNAzyme R1 increase intracellular stability in mammalian tissue culture and allow for mRNA cleavage. Fluorescence image of HEK293T cells co-transfected with DsRed and GFP expressing plasmids and the DNAzymes R1 (non-caged) and R2 (caged at T37). A) Transfection of the non-caged DNAzyme R1 leading to the silencing of DsRed expression. B) Transfection of the DNAzyme R2 caged at the essential residue T37 in the catalytic core, previously shown to abrogate DNAzyme activity; however, in this case DNAzyme complete silencing of DsRed is still observed. C) Control DNAzyme R7 transfection leading to the expression of both DsRed and GFP. Scale bar = 200 μm.