Inhibition of replication of HIV, SIV and SHIV in C46-expressing cell lines. PM-1 cells were transduced with retroviral vectors encoding for the antiviral maC46 sequence derived from HIV, SIV, or SHIV (A, D, and G: M87oHIV; B, E, and H: M87oSHIV; C, F, and I: M87oSIV). PM-1 cells transduced with the inactivate form of the C46 peptide were used as control cells. PM-1 cells were infected with HIV-1 NL4-3 (A-C), SIVmac251 (D-F), or SHIV89.6P (G-I) and the amount of p24 or p27 antigen in culture supernatants was determined by ELISA. The data shown are representative graphs from three experiments that were each performed in duplicate.