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. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: Gene Ther. 2008 May 1;15(17):1210–1222. doi: 10.1038/gt.2008.73

Figure 3.

Figure 3

Efficacy of entry inhibition of HIV, SIV and SHIV afforded by low and high maC46-expressing variants of retroviral vectors. PM-1 cell lines expressing high levels of maC46 were generated by fluorescence activated cell sorting. PM-1 cell lines with a low expression of maC46 were generated by selection with G418. Control PM-1 cells were transduced with a retroviral vector coding for IRES-Neo and selected with G418. (A) Expression level of maC46 was detected by 2F5 mAb staining. (B-D) Relative level of transduction compared to control PM-1 cells. Lentiviruses coding for eGFP were either pseudotyped with HIV-1 JRFL, SIVmac251 or SHIV89.6P envelope protein. Transduction efficacy was determined by measuring eGFP expression by flow cytometry.