Skip to main content
. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: Gene Ther. 2008 May 1;15(17):1210–1222. doi: 10.1038/gt.2008.73

Figure 5.

Figure 5

Expansion, M87oHIV transduction and phenotype of maC46-transduced CD4+ T cells. CD8+ lymphocyte-depleted and total lymphocytes obtained from peripheral blood were transduced with the M87oHIV retroviral vector and cultured with anti-CD3 and anti-CD28 coated beads for 7 days. (A) Rate of expansion of CD4+ T cells 7 days after initiation of in vitro culture. Cell counts were determined by hemacytometer. The black bar represents the bulk culture, the white bar the CD8+ cell-depleted culture. (B) Transduction efficacy of CD4+ T cells with the retroviral vector M87oHIV. The cells were transduced twice with a multiplicity of infection (MOI) of 1.3-2 or more than a MOI of 3.3. M87oHIV expression was determined by staining with a C46 specific mAb. The black bar represents the bulk culture, the white bar the CD8+ cell-depleted culture. (C) and (D) Phenotype of expanded CD4+ T cells. On day 7 after culture, cells were stained with anti-CD28 and anti-CD95 mAbs to determine T cell subsets and with 2F5 to determine C46 expression. The black bars represent M87oHIV-expressing cells, the white bars M87oHIV negative cells. The bars in all graphs represent median values, error bars the interquartile range (n = 4-10).