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. Author manuscript; available in PMC: 2010 May 3.
Published in final edited form as: Gene Ther. 2008 May 1;15(17):1210–1222. doi: 10.1038/gt.2008.73

Figure 7.

Figure 7

Effect of different cytokines on efficacy of expansion and level of transduction of CD4+ T cells. PBMC were depleted of CD8+ cells and expanded for 7 days with anti-CD3 and anti-CD28-coated paramagnetic beads. The different cytokines or combinations of cytokines were added on day 0. Cell culture supernatants were partially replaced with fresh media containing the different cytokines on days 4, 5 and 6. On days 4 and 5 cells were transduced with M87oHIV retroviral vectors. (A) Fold expansion of CD4+ T cells expanded in different cytokines or cytokine combinations relative to IL-2 treated cells that were set to an expansion factor of 1. (B) M87oHIV transduction efficacy of CD4+ T cells expanded in different cytokines or cytokine combinations. The bars represent median values from 5 animals. (C-F) Side and Forward Scatter of one representative dot plot of CD4+ T cells expanded in media without cytokines (C), supplemented with IL-2 (D), IL-7 (E), and IL-7 + IL-15 (F).

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