Figure 3. High light intensity suppresses DN1-generated evening locomotor activity and temperature modulates DN1-generated morning and evening behavior.
Locomotor activity of adult male flies measured during 3 days of LD cycle under different light intensities and temperatures and averaged. (A) 500 lux, 25°C. (B) 50 lux, 25°C (C). 500 lux, 20°C. Arrows indicate the morning anticipation (black) and evening anticipation (white). Dots above error bars correspond to Standard Errors of the Mean. Genotypes in (A), (B) and (C): w1118 (upper panels), per01;;ry506 (middle panels) per01 w;; Clk4.1M-GAL4/UAS-per 16. (lower panels). See also Figure S2 for additional controls and quantifications.