FIG. 2.
Thicknesses of epidermal layers on collagen–glycosaminoglycan (GAG) membranes. Keratinocytes were cultured for 3 or 7 days at the air/liquid A/L interface on collagen–GAG control (nonmodified) membranes or collagen–GAG membranes that were modified by passively adsorbing fibronectin (FN) to the surfaces of the scaffolds. (A) Histological representation of grafts at 3 or 7 days of A/L interface culture. The thickness of the epithelial layer on collagen–GAG membranes treated with FN was greater than that on untreated collagen–GAG membranes. Scale bar represents 30 μm. (B) Epidermal thickness on collagen–GAG membranes at 3 or 7 days of A/L interface culture was measured on control (nonmodified) collagen–GAG membranes or collagen–GAG membranes that were modified by passively adsorbing FN to the surfaces. At both 3 and 7 days there was a significant difference between untreated and FN-treated surfaces (*p < 0.05, Student's t-test). Samples for 3-day culture are n = 7 and for 7-day culture n = 4. Color images available online at www.liebertonline.com/ten.
