FIG. 3.

Proliferation of keratinocytes on collagen–GAG membranes. Keratinocytes were cultured on collagen–GAG membranes for 3 or 7 days at the A/L interface on control (nonmodified) collagen–GAG membranes or collagen–GAG membranes modified by passively adsorbing FN to the surfaces. (A) Histological representation of grafts at 3 or 7 days of A/L interface culture immunostained for Ki67 (brown stained nuclei) to evaluate proliferation of basal keratinocytes. Scale bar represents 30 μm. (B) The percentage of positive Ki67 basal keratinocytes at 3 or 7 days of A/L interface culture was measured on control (nonmodified) collagen–GAG membranes or collagen–GAG membranes modified by passively adsorbing FN to the surfaces. At 3 days statistical differences were found between keratinocytes cultured on control surface and FN-treated surfaces (*p < 0.05, Student's t-test). For all experimental conditions, n = 5 samples were measured at both 3 and 7 days of culture. Color images available online at www.liebertonline.com/ten.