FIG. 4.

Availability of cellular binding domains for FN passively adsorbed on collagen–GAG basal lamina analogs. The availability of the cellular binding domain of FN on collagen–GAG basal lamina analogs was evaluated using a quantitative immunofluorescent assay. FN concentrations of 0, 30, 100, and 300 μg/mL were evaluated, and it was determined that at a concentration of 100 μg/mL the average relative fluorescence intensity (RFI) was statistically different from 0 μg/mL and 30 μg/mL, but did not statistically differ from 300 μg/mL, indicating that a saturation plateau was achieved. Data are reported as averages, and error bars indicate standard error of the mean with n = 3 (*statistical difference, one-way analysis of variance [ANOVA] with Tukey post hoc analysis, p < 0.05).