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Cell encapsulation and self-assembly mold. The acelluar-agarose annulus mold is made by injecting 2% agarose into a custom, autoclavable frame. Fifty microliters of cell-hydrogel suspension (bone marrow stromal cells [BMSCs] in RAD16-I, KLD12, or agarose) is then injected into the center to initiate self-assembly.
