Representative Toluidine Blue staining for (A) sulfated glycosaminoglycan, and (B) collagen type II and (C) type I immunohistochemistry images for BMSCs cultured with TGF-β1 supplementation in agarose (left column), RAD16-I (center column), and KLD12 (right column). (See “Materials and Methods” for details.) Culture duration was 21 days. (D) Collagen types I and II immunohistochemistry in bovine cartilage–bone plugs as controls. Scale bar in (A) is applicable to (B), (C), and (D), in all cases scale bar = 50 μm. Color images available online at www.liebertonline.com/ten.