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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Dev Dyn. 2009 Nov;238(11):2713–2724. doi: 10.1002/dvdy.22097

Figure 2. MMP2 gene expression and activity is elevated in response to PTHrP and BMP treatment.

Figure 2

Figure 2

A) qPCR results for C3H10T1/2 cells treated with PTHrP, BMP4 or the combination of PTHrP and BMP4. Bars represent the average and SEM of three independent experiments. * denotes statistical significance as compared to no treatment (NT) by ANOVA (P < 0.05). # denotes that treatment with BMP4 was significantly different than NT when a direct comparison was made using a one-sample t test (P = 0.013). B) MMP activity in conditioned media from C3H10T1/2 cells treated with PTHrP, BMP4 or the combination of PTHrP and BMP4. MMP2 activity was measured by densitometric evaluation of gelatin zymography. Each bar represents the average and SEM of 8–10 individual experiments. Statistical significance was assessed by ANOVA employing the Newman-Keuls multiple comparison test. * denotes statistical significance as compared to NT (P < 0.001). # denotes statistical significance as compared to PTHrP (P < 0.001). § denotes statistical significance as compared to BMP4 (P < 0.05).