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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3060–3073. doi: 10.1021/bi901719e

Figure 5. Comparison of the electrogenic protonic phases in N139L, N139D and wild type cytochrome oxidase.

Figure 5

Experiments with collodion film-adhered liposomes with COX have been carried out in the buffer containing 5 mM Tris-acetate, pH 8, with 40 μM RuBpy and 10 mM aniline as the photoreducing system. 2 mM H2O2 was added to convert COX to the ferryl-oxo state prior to the flash. The traces have been normalized by the magnitude of the microsecond KCN-insensitive part of the response (indicated by a dashed line) and contribution of the membrane passive discharge has been subtracted from all the curves (cf. Figures S4, S5).