Figure 7. The kinetics of rapid phase of membrane potential generation does not match the kinetics of heme a reduction during single-electron photoreduction of Compound F in N139L oxidase.
Conditions for the absorption measurements, as in the Figures 4, S5; for the electrometric measurements, as in Figures 5,6. The amplitude of ΔΨ generation is 0.75 mV for the electrometric trace and the maximal value of ΔA445 for the absorption trace is 0.0067 O.D.