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. 2010 May 3;5(5):e10436. doi: 10.1371/journal.pone.0010436

Figure 1. Upregulation of GITR expression correlates with optimal timing of single dose DTA-1 therapy.

Figure 1

A and B. C57BL/6 mice (n = 10/group) were challenged intradermally with 50,000 B16 melanoma cells and treated with 1 mg DTA-1 or rat IgG i.p. on day 4 after tumor challenge. Tumor survival (A) and mean tumor diameter + SEM over time is depicted (B) C. Untreated mice (n = 3/group) bearing 4 day-old B16 matrigel tumors (500,000 cells) were sacrificed and lymphocytes isolated from spleens (S), tumor-draining lymph nodes (DLN), and tumors (T), were stained for CD4, CD8, foxp3, and GITR. Mean GITR fluorescence intensity (MFI) and SEM within each T cell subset is depicted.