Figure 4. Withdrawal and withdrawal relief can be observed at shorter periods of ethanol conditioning.

Developmentally staged worms (L4 plus 1 day) were conditioned on abundant food in the absence (naive) or presence (withdrawn) of 354±32 mM ethanol for 6 h, recovered from the plates by washing and then tested in the food race either in the absence (naive, withdrawn) or presence (60±1 mM; naive + low eth, withdrawn + low eth) of ethanol. Each point is the mean ± s.e.mean of 4 independent determinations for 50 worms in a food race. Statistical analysis was performed using one way ANOVA with Bonferroni multiple comparisons post-test on the last time-points. ** p<0.01.