Skip to main content
. 2010 May 3;5(5):e10438. doi: 10.1371/journal.pone.0010438

Figure 5. ACE activity of mutant ACE.

Figure 5

ACE activity of the membrane-bound form of ACE in lysates of CHO cells expressing WT and mutant ACE was determined by fluorimetric assay of 40 µl aliquotes of cells with 200 µl each of substrates Hip-His-Leu (HHL) and Z-Phe-His-Leu (ZPHL) during 1 hr incubation. A. ACE activity with HHL and ZPHL (mU/ml), correspondingly, from a representative experiment performed in triplicate. B. Ratio of hydrolysis of the two substrates (ZPHL/HHL ratio) in the tested samples. The data are presented as mean (± SD) from three independent experiments. *p<0.05 vs. control samples.