Figure 10. Chaperone-like effect of anti-ACE mAbs on mutant ACE.

Membrane-bound (A) and soluble (B) mutant ACE (Q1069R) were pre-incubated with N-domain specific mAb 9B9 [30] or C-domain specific mAbs 1B3 and 1B8 [16], [36] at the indicated concentrations for 2 hrs at room temperature and then residual activity was determined with 5 mM HHL and 2 mM ZPHL as substrates. Experimental conditions are as described in the legend to Fig. 5. Data presented as ZPHL/HHL ratio of ACE activity in the presence mAb. Results shown are the mean ± SD of 3–4 experiments.