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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mol Immunol. 2010 Jan 29;47(6):1244–1254. doi: 10.1016/j.molimm.2009.12.013

Figure 2.

Figure 2

β-AR activation synergistically increases LPS initiated production of IL-1β in THP-1 cells. Panel A, Representative immunoblot for equal amounts of total resolved protein from THP-1 cell lysates individually treated with 30 ng/mL LPS, 0.1 μM Iso or concurrently with both, then subsequently probed with an IL-1β antibody as outlined in the “Materials and Methods”. There was a qualitative increase in pro-IL-1β (33 kDa) production from lysates treated with LPS plus Iso when compared to Iso only or LPS alone. Panel B, Semi-quantitative analysis of all immunoblots (n = 5) showed a significant (p < 0.05) increase in the expression of IL-1β from lysates treated with LPS (12.1 ± 3.9 fold) when compared to basal (*). In addition, there was a significant (p < 0.05) synergistic production of IL-1β from lysates incubated with LPS plus Iso (45.2 ± 15.8 fold over basal) when compared to LPS alone (#). There were no significant differences in the amount of IL-1β generated from lysates treated with Iso (3.1 ± 0.8 fold) when compared to basal. Panel C, Amounts of secreted IL-1β quantified from the media of treated THP-1 cells using ELISA analysis (n = 6). Significant (p < 0.05) increases were found in the amount of IL-1β secreted (16.9 ± 2.6 pg/mL) from cells treated with LPS when compared to control (*). In addition, cells treated with LPS plus Iso demonstrated a synergistic increase in IL-1β (76.3 ± 24.7 pg/mL), which was significantly (p < 0.05) different from amount secreted from LPS only (#). There were no changes in the amount of secreted IL-1β from cells treated with 0.1 μM Iso (4.8 ± 1.4 pg/mL) when compared to control (1.6 ± 0.5 pg/mL).