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. 2010 May 3;5(5):e10475. doi: 10.1371/journal.pone.0010475

Figure 2. ACF carpet analysis.

Figure 2

(A) A 3.2 µm-line across NE is scanned left-to-right with a pixel dwell time of 6.3 µs (32 pixels) and a line time of 0.47 ms. Scale bar of the image: 5 µm. (B) Fluorescence intensity is represented as a carpet with the line in the x-direction and the time in the y-direction. (C) The ACF is calculated pixel-by-pixel along the scanned line and represented as a carpet. (D) Two columns extracted from the ACF carpet are displayed, one corresponding to the nucleus (col. 3, in red), the other to the cytoplasm (col. 28, in blue). (E) 3-D-plot of the total ACF carpet.