Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Mar 27;42(6):1039–1045. doi: 10.1016/j.biocel.2010.03.014

Fig.1. AR inhibition prevents LPS-induced glucose uptake in macrophages.

Fig.1

Untransfected and AR-siRNA transfected RAW264.7 cells were incubated with and without 2μM fidarestat and iNOS inhibitor (1400W) for 24 h and 30 min, respectively followed by incubation with 1μg/ml LPS. After 6 h of LPS treatments cells were replaced with fresh medium containing C14 labeled methyl-D-glucose for respective time intervals. At the end of the incubations, cells were washed twice with ice cold PBS and cell contents were dissolved in 0.1N NaOH for overnight and neutralized with hydrochloric acid. Equal amount of cell extracts were subjected for radioactivity measurements using scintillation counter. All the data are expressed as Mean ± SEM (N = 4). *P < 0.001 as compared to control cells (0 min), #P < 0.001 compared to LPS-treated respective groups.