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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Mar 27;42(6):1039–1045. doi: 10.1016/j.biocel.2010.03.014

Fig.2. AR inhibition prevents LPS-induced activation of glucose transporters.

Fig.2

Growth-arrested mouse macrophages (RAW264.7) were incubated with and without 2 μM fidarestat for 24 h and cells were treated with LPS for various time points ranging from 0-24 h. A) whole cell lysates and B) membrane fractions were probed against glucose transporter protein antibodies GLUT-1, GLUT-3 and GLUT-4. C) RT-PCR was performed for GLUT-3 expression using total RNA isolated from treated macrophages as described in methods. Band intensities were measured by densitometry analysis to determine the fold changes. Data shown are representative of three independent experiments.