Figure 6. Results of Extra pulses of bioflavonoid compounds and reversal of inhibition by passing through centrifuge columns.

(A), Membrane bound ATP synthase (Mbr) or purified F₁ (F₁) was inhibited with inhibitory concentrations of the bioflavonoid compounds shown in the figure for 60 min under conditions as described in Fig 3–4. Then a further pulse of identical inhibitory concentrations was added and incubation continued for 1 h before assay. The last digits represent the compound concentrations in [μM]. (B) Purified F₁ was incubated with inhibitory concentrations of bioflavonoid compounds for 60 min under conditions as described in Fig 2–4. Then the inhibited samples were passed twice through 1 ml centrifuge columns and ATPase activity was measured. The first bar is for purified F₁ with no compound (F1), followed by bars in presence of compounds.