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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Matrix Biol. 2010 Jan 22;29(4):304–316. doi: 10.1016/j.matbio.2010.01.005

Figure 4. Abnormal aortic valves are seen in Adamts9+/LacZ hearts.

Figure 4

WT (A) and Adamts9+/LacZ heterozygous mouse heart sections (B–E) were stained with the Movat pentachrome stain. Abnormal aortic leaflets (B–E) with an increase in blue staining indicative of an increase in proteoglycans are shown (B, C and D, blue staining overlapped with versican immunoreactivity, data not shown). Quantification of the area of adult WT (n=6) and Adamts9+/LacZ heterozygous (n=8) aortic valves is shown in panel F. Area measurements were performed in frontal sections (e.g. Figure 2A and Figure 8A–C) through the widest point of the aorta spanning a minimum of 40 μm width in 9 Adamts9+/LacZ hearts and 3 WT hearts. The total area of valves in pixels was compared and the P value determined by Student t-test (2 tailed, type 2) was P = 6.7 × 10−07. The penetrance of the aortic valve phenotype was 8/15 at 53%. The Adamts9+/LacZ hearts with the aortic valve phenotype were analyzed in this analysis. The Y axis represents the total number of positive pixels of the semilunar valves measured. Magnification bar in A = 200μm applies to B–E.