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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Matrix Biol. 2010 Jan 25;29(4):261–275. doi: 10.1016/j.matbio.2010.01.006

Fig. 10.

Fig. 10

Spatial distribution of clade B procollagen gene expression in caudal regions of 30, 48 and 72 hpf embryos. (A) Whole mount in situ hybridization shows expression of col11a1b, col11a1a and col11a2, and col2a1 as a control, in the notochord of 30 (a-d), 48 (e and f), and 72 (g-i) hpf embryos. (B) Whole mount in situ hybridization shows expression of col5a1, col5a3a and col1a1 as a control, in the notochord of 30 (a-d), 48 (e and f) and 72 (g and h) hpf embryos. Note similar localization of col5a1 and col1a1 expression surrounding the notochord and between myotomes at 48 and 72 hpf (panels eh). Col1a1 (arrowhead), but not col5a1, expression, is strong in the caudal fin fold. A probe corresponding to Col5a3a exons 1-5 and 7 sequences detected expression at 30 hpf throughout the length of the notochord (panel b), whereas a probe corresponding to alternatively spliced Col5a3a exon 6 (splice) only detected expression in the caudal notochord (panel c) at this time.