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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Trends Cell Biol. 2010 Mar 18;20(5):277–286. doi: 10.1016/j.tcb.2010.02.003

Figure 2. Acidocalcisomes in whole cells and subcellular fractions.

Figure 2

(a), (b), ultrathin sections of Agrobacterium tumefaciens (a) or Trypanosoma brucei procyclic forms (b) showing the acidocalcisomes as empty vesicles with electron-dense inclusions (arrows). (c), (d), visualization of acidocalcisomes in whole unfixed Herpetomonas anglusteri and Trypanosoma cruzi acidocalcisome fraction obtained by iodixanol centrifugation [6] allowed to adhere to Formvar- and carbon-coated grids and then observed by direct transmission electron microscopy (using an energy filter in (c)).Acidocalcisomes (black granules) appear disperse in the cytoplasm (c) or fraction (d). Scale bars: (a), 0.2 μm; (b), 2 μm; (c), 0.5 μm; (d), 1 μm. (a) was taken by Manfredo Seufferheld. (c) is reproduced with permission from ref. [114].