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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Cells. 2010 Mar 4;29(4):333–341. doi: 10.1007/s10059-010-0029-8

Figure 4. IKKβ is active in v-Abl-transformed cells and inhibits FoxO3a activity.

Figure 4

a) 7G-S cells were cultured in the presence of 2 μM AKT inhibitor VIII, 10 μM STI571, or 5 μM IKKβ inhibitor III for 20 hours at which time TRAIL and HPRT transcripts were analyzed by real-time PCR. TRAIL transcripts were normalized to HPRT. Graph presents fold-induction of TRAIL.

b) myr-Akt-ER-transduced cells were cultured in the presence of 4 μM OHT, 5 μM IKKβ inhibitor III, or both for 17 hours at which time transcripts levels of TRAIL and HPRT were determined by real time PCR. Results indicate fold TRAIL induction.

c) Total cell lysates from 7G-S Abelson cells cultured in the presence of 10 μM STI or 10 μM IKKβ inhibitor III, and analyzed by Western blot for FoxO3a and actin.

d) The graph represents the intensity of the FoxO3a band normalized to the intensity of the actin band from two independent repetitions of the experiment shown part c, above.