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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Int J Cancer. 2010 Jul 1;127(1):239–244. doi: 10.1002/ijc.25012

Figure 2. Tumor induction and angiogenesis induced by PIP2 affine AKT1 constructs in the CAM of the chicken embryo.

Figure 2

CAM assays were performed with fertilized chicken eggs (White Leghorn) obtained from Charles Rivers Breeding Laboratories (Wilmington, MA). Eggs were incubated at 37.5 °C and 70% humidity for 9 days. Eggs were candled to confirm embryo growth and to locate vascularized areas of the CAM. Two holes were drilled, one into the air sac at the bottom of the eggs, another one at the lateral end above the blood vessels, penetrating through the outer shell membrane without injuring the CAM. Applying suction to the air sac exposed the CAM. CAMs were inoculated with AKT1, E17K-AKT1, myrAKT1, GRP-AKT1, PLC-AKT1 or empty vector. Eggs were sealed and incubated for another 9 days. A representative experiment out of three independent ones is shown.