Figure 7.
Expression of As3mt in cultured primary hepatocytes from dog, macaque, rat, rabbit, mouse, and human: Analysis of As3mt mRNA by RT-PCR (a); As3mt protein detected by rabbit anti-serum against recombinant rat As3mt (b); As3mt protein detected by rabbit polyclonal antibody against human AS3MT diluted 1:5,000 (c) and 1:2,000 (d); immunoblot analysis of β-actin (e); As3mt protein level detected by the rabbit anti-serum normalized for β-actin (b/e); As3mt protein levels detected by the rabbit polyclonal antibody normalized for β-actin (c/e).